Journal: Cell reports

Article Title: Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration

doi: 10.1016/j.celrep.2020.107572

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: A5441; RRID:AB_476744 HRP Goat Anti-Rabbit IgG Vector Labs Cat. No. PI-1000; RRID:AB_2336198 HRP Horse Anti-Mouse IgG Vector Labs Cat. No. PI-2000; RRID:AB_2336177 Bacterial and Virus Strains TCF4-shRNA (E2-2) Sigma-Aldrich SHCLNG- {"type":"entrez-nucleotide","attrs":{"text":"NM_013685","term_id":"145386571","term_text":"NM_013685"}} NM_013685 (TCRN0000012093, TCRN0000012094) MISSION TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid DNA Sigma-Aldrich Cat. No. SHC202 BCL2L1-pLX307 Addgene Cat. No. 98323 pLEX_307 Addgene Cat. No. 41392 Chemicals, Peptides, and Recombinant Proteins poly(I:C) InVivogen Cat. No. tlrl-pic-5 Tamoxifen Sigma-Aldrich Cat. No. T5648 Corn Oil Sigma-Aldrich Cat. No. C8267 DPBS, no calcium, no magnesium Thermo Fisher Scientific Cat. No. 14190250 Bovine Serum Albumin Sigma-Aldrich Cat. No. A4503-100G Lymphocyte Separation Medium Lonza Cat. No. 17-829E ACK Lysis Buffer Lonza Cat. No. 10-548E Trypan Blue Solution, 0.4% Methyl Thermo Fisher Scientific Cat. No. 15250061 Paraformaldehyde Fisher Scientific Cat. No. T353-500 Cytofix/Cytoperm and Perm/Wash Buffers BD Biosciences Cat. No. 554714 FxCycle Violet Dye Thermo Fisher Scientific Cat. No. {"type":"entrez-nucleotide","attrs":{"text":"F10347","term_id":"683005","term_text":"F10347"}} F10347 Propidium Iodide Thermo Fisher Scientific Cat. No. BMS500PI Amoxicillin Covetrus North America Cat. No.060851 Enrofloxacin Covetrus North America Cat. No. NDC55529-154-11 Decalcifying Solution-Lite Sigma-Aldrich Cat. No. D0818 Sucrose Sigma-Aldrich Cat. No. 84097 OCT compound Fisher Scientific Cat. No. 12-730-571 Blocking Reagent Sigma-Aldrich Cat. No. 11096176001 Triton X-100 Sigma-Aldrich Cat. No. T8787 DAPI Thermo Fisher Scientific Cat. No. D103 Tween 20 Sigma-Aldrich Cat. No. P1379 Dako Fluorescence Mounting Medium Agilent Cat. No. S3023 Benzyl Alcohol Sigma-Aldrich Cat. No. 305197 Benzyl Benzoate Sigma-Aldrich Cat. No. B6630 Immersol 518F Immersion Oil Fisher Scientific Cat. No. 10539438 Glutaraldehyde Tousimis Cat. No. 1015 Sodium Cacodylate Buffer Electron Microscopy Sci.

Techniques: Plasmid Preparation, Virus, shRNA, Control, Recombinant, Lysis, Blocking Assay, Fluorescence, Electron Microscopy, In Situ, Flow Cytometry, SYBR Green Assay, Bicinchoninic Acid Protein Assay, RNA Sequencing, Software, Microscopy